- PII
- S0006302925010101-1
- DOI
- 10.31857/S0006302925010101
- Publication type
- Article
- Status
- Published
- Authors
- Volume/ Edition
- Volume 70 / Issue number 1
- Pages
- 86-92
- Abstract
- α -Subunits of the potassium voltage-gated channel Kv1.2, whose function is to regulate neuronal conductivity in the central nervous system, form heterotetramers with α-subunits of related Kv1 channels, which differ in composition and stoichiometry. To study heterotetrameric channels in vitro, concatemers are constructed by sequentially connecting the Kv1 α-subunits. The method for constructing concatemers that allows one to reproduce the properties of native channels requires detailed study. In this work, concatemers (dimers) of Kv1.2 α-subunits (mKate2-Kv1.2-Kv1.2) labeled with the fluorescent protein mKate2 were constructed Kv and their expression was carried out in mouse neuroblastoma Neuro-2a cells. It was shown that the Kv1.2 channel assembled from concatemers is almost identical in its properties, namely, intracellular distribution, ability to integrate into the plasma membrane, efficiency of interaction with a peptide blocker, as well as in its electrophysiological characteristics, to the Kv1.2 channel based on monomeric α-subunits mKate2-Kv1.2.
- Keywords
- калиевые каналы потенциал-зависимые гетеротетрамеры пептидные блокаторы электрофизиология конфокальная микроскопия
- Date of publication
- 24.10.2025
- Year of publication
- 2025
- Number of purchasers
- 0
- Views
- 18
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