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RAS BiologyБиофизика Biophysics

  • ISSN (Print) 0006-3029
  • ISSN (Online) 3034-5278

Identification of Potential SOX2 Binding Sites to Nucleosomes via Molecular Modeling

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PII
S30345278S0006302925030039-1
DOI
10.7868/S3034527825030039
Publication type
Article
Status
Published
Authors
T.A. Romanova
  • Affiliation: Lomonosov Moscow State University
D.M. Ryabov
  • Affiliation: Lomonosov Moscow State University
G.A. Komarova
  • Affiliation: Lomonosov Moscow State University
A.K. Shaytan
  • Affiliation: Lomonosov Moscow State University
G.A. Armeev
  • Affiliation: Lomonosov Moscow State University
Volume/ Edition
Volume 70 / Issue number 3
Pages
439-452
Abstract
The pioneer transcription factor SOX2 plays an important role in the regulation of gene expression by binding to condensed chromatin and inducing its decondensation. Experimental data on the preferred positions of SOX2 binding to DNA in the context of the nucleosome are contradictory: in some studies, binding to the inner segments of nucleosomal DNA is preferred, in others – to the edge segments. Within the framework of this work, all possible variants of SOX2 binding to nucleosomal DNA at different distances from the nucleosome center (determined by the superhelix position parameter – SHL) and different orientation of the binding site relative to the nucleosome center (determined by the SHL sign) were analyzed by molecular modeling. It was shown that on an intact nucleosome, binding is possible at positions SHL +2, SHL ±4 and SHL ±5, but if we assume a shift of nucleosomal DNA by one pair of nucleotides, binding becomes possible at positions corresponding to all integer values of SHL. This observation helps to explain part of the contradictions between the experimental data.
Keywords
хроматин нуклеосома пионерные транскрипционные факторы молекулярное моделирование
Date of publication
07.05.2025
Year of publication
2025
Number of purchasers
0
Views
47

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