- PII
- S30345278S0006302925040145-1
- DOI
- 10.7868/S3034527825040145
- Publication type
- Article
- Status
- Published
- Authors
- Volume/ Edition
- Volume 70 / Issue number 4
- Pages
- 749-756
- Abstract
- The genotoxicity of sodium nitroprusside on human MCF-7 mammary gland adenocarcinoma cells was studied using the neutral comet method and the contribution of the nitrosonium ion (NO+) to the genotoxicity of sodium nitroprusside was determined. Genotoxicity was assessed by the yield of double-stranded DNA breaks after incubation of a cell culture with the agent for 1.5 hours. The genotoxicity of NO+ in sodium nitroprusside was determined after the procedure of reducing sodium nitroprusside using sodium dithionite, which converts nitroprusside from a NO+ donor to a NO only donor. It has been shown that sodium nitroprusside induces double-stranded DNA breaks in MCF-7 cells after 1.5 hours. This ability of nitroprusside does not depend on the stage of the cell cycle: the proportions of damaged cells in the G0/G1 and G2/M+S stages are 29 ± 7% and 27 ± 6%, respectively. As a result of 90-minute incubation with 100 μM of sodium nitroprusside, the proportion of cells severely damaged in the form of double-stranded DNA breaks is 25−30%, which is close to the proportion of non-viable cells according to the results of the MTT test at 24 h and 48 h of incubation (22 ± 4%). Sodium dithionite, by removing the toxic effect of NO+, protected MCF7 cells from the genotoxic effects of nitroprusside, which indicates the involvement of NO+ in the formation of double-strand breaks.
- Keywords
- нитропруссид катион нитрозония одно- и двунитевые разрывы ДНК оксид азота генотоксичность динитрозильные комплексы железа
- Date of publication
- 16.12.2025
- Year of publication
- 2025
- Number of purchasers
- 0
- Views
- 50
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