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RAS BiologyБиофизика Biophysics

  • ISSN (Print) 0006-3029
  • ISSN (Online) 3034-5278

Structure and Cooperative Interactions Between the Guanine Quadruplexes of the Promoter of β-Globin Gene

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PII
S30345278S0006302925050021-1
DOI
10.7868/S3034527825050021
Publication type
Article
Status
Published
Authors
E.V. Marilovtseva
  • Affiliation: The Mental Health Research Center
D.O. Koshkina
  • Occupation: Faculty of Biology
  • Affiliation:
    Lomonosov Moscow State University
    Institute of Gene Biology, Russian Academy of Sciences
A.V. Feofanov
  • Occupation: Faculty of Biology
  • Affiliation: Lomonosov Moscow State University
V.M. Studitsky
  • Occupation: Faculty of Biology
  • Affiliation:
    Lomonosov Moscow State University
    Fox Chase Cancer Center
Volume/ Edition
Volume 70 / Issue number 5
Pages
854-863
Abstract
Guanine quadruplexes are nucleic acid secondary structures present in the genomes of all eukaryotes, from yeast to mammals, where they play an important role in maintaining telomere integrity, creating TAD boundaries, and regulating transcription, alternative splicing, and translation. It was found that, contributing to the formation of a nucleosome-free region, guanine quadruplexes formed by two G-rich motifs inside the replication origin that is located within the β-globin promoter in , are necessary for the initiation of replication. In our work, circular dichroism spectroscopy was used to study the structures and dynamic properties of guanine quadruplexes formed by the β-globin promoter/origin sequence . The data obtained show that quadruplexes located on the same DNA fragment are formed cooperatively, influencing the structures of each other and the entire DNA fragment on which they are located. These data suggest that the structures of guanine quadruplexes may be determined by their genomic environment, and also help explain some of the properties of quadruplexes observed .
Keywords
гуаниновый квадруплекс нуклеосома βA-глобин ориджин репликации спектроскопия кругового дихроизма
Date of publication
13.12.2025
Year of publication
2025
Number of purchasers
0
Views
25

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