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RAS BiologyБиофизика Biophysics

  • ISSN (Print) 0006-3029
  • ISSN (Online) 3034-5278

Complexation of Papain with Particles of Chitosan and Carboxymethylchitosan, Obtained in the Presence and in the Absence of Ascorbic Acid

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PII
S0006302925010036-1
DOI
10.31857/S0006302925010036
Publication type
Article
Status
Published
Authors
M. G Holyavka
  • Affiliation:
    Voronezh State University
    Sevastopol State University
M. V. Kozlova
  • Affiliation: Voronezh State University
Volume/ Edition
Volume 70 / Issue number 1
Pages
22-34
Abstract
Particles of medium and high molecular weight chitosans and carboxymethylchitosans were obtained without and with the addition of ascorbic acid. Methods were developed to obtain complexes of these particles with the plant protease papain. It was found that the activity of papain complexes with particles of carboxymethyl chitosan is significantly higher compared to its complexes with particles of chitosan. At the same time, complexes with particles of chitosan and carboxymethyl chitosan obtained with the addition of ascorbic acid showed higher values of proteolytic activity than complexes with polysaccharide particles obtained without its addition. Using molecular docking, it was found that amino acid residues of papain interact with both chitosan and carboxymethyl chitosan mainly by forming hydrogen bonds and van der Waals interactions. In addition, ascorbic acid molecules and both types of polysaccharides interact with amino acid residues from the active site of papain – Cys25 and His159, which probably contributes to an increase in the activity and stability of the enzyme in complexes with particles of chitosan and carboxymethyl chitosan, including due to the fact that ascorbic acid prevents the oxidation of the thiol group of papain, which is responsible for the act of catalysis.
Keywords
папаин хитозан карбоксиметилхитозан комплексообразование
Date of publication
24.10.2025
Year of publication
2025
Number of purchasers
0
Views
17

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