- PII
- S0006302925010082-1
- DOI
- 10.31857/S0006302925010082
- Publication type
- Article
- Status
- Published
- Authors
- Volume/ Edition
- Volume 70 / Issue number 1
- Pages
- 72-77
- Abstract
- The major goal of this work was to study the stoichiometry of the interaction of cytochrome C with tetraoleoyl- or tetramyristoyl-cardiolipin. Complexes of cytochrome C with phospholipid play one of the main roles in apoptotic processes. This study was conducted using fluorescently labeled phosphatidylcholine (BODIPY-PC). The fluorescence quenching of the BODIPY-PC probe after its interaction with cytochrome C was measured. Calculations of the fluorescence quenching results allowed us to determine the stoichiometry of the cytochrome C complexes formed with phospholipids. For complexes containing tetraoleoyl, stoichiometry corresponded to the ratio tetraoleoyl : cytochrome C equal to 25 ± 7, and for tetramyristoyl the ratio tetramyristoyl : cytochrome C – 7 ± 2. Obtained results may indicate a difference in the affinity of cytochrome C for saturated and unsaturated types of cardiolipin, despite the same electrostatic characteristics of the molecules. It can be expected that the discovered features of the interaction of cytochrome C with cardiolipin will eventually help determine the intensity and direction of apoptotic reactions.
- Keywords
- апоптоз цитохром с кардиолипин комплекс цитохрома с с кардиолипином флуоресцентно-меченый BODIPY фосфатидилхолин
- Date of publication
- 24.10.2025
- Year of publication
- 2025
- Number of purchasers
- 0
- Views
- 20
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