Selenium as a Protector Against Hydrogen Peroxide Oxidative Degradation of Heme of Hemoglobin without the Glutathione Peroxidase Mechanism

Huseynov, T. M; Kozlova, M. V.

doi:10.31857/S0006302925010124

PII

S0006302925010124-1

DOI

10.31857/S0006302925010124

Publication type

Article

Status

Published

Authors

T. M Huseynov

Affiliation: Institute of Biophysics, Ministry of Science and Education of Azerbaijan Republic

M. V. Kozlova

Affiliation: Institute of Biophysics, Ministry of Science and Education of Azerbaijan Republic

Volume/ Edition

Volume 70 / Issue number 1

Pages

104-111

Abstract

The protective effect of sodium selenite (Na2SeO3) on the oxidative degradation of hemoglobin induced by hydrogen peroxide (H2O2) was studied by recording the resulting two fluorescent products of heme breakdown (λex = 321 nm, λem = 460 nm) and (λex = 465 nm, λem = 525 nm). It has been established that sodium selenite (Na2SeO3) inhibits the development of oxidative modification of hemoglobin (depletion of oxyhemoglobin, accumulation of methemoglobin and ferrylhemoglobin), which is reflected in a noticeable 20–30% decrease in fluorescence peaks, reflecting the oxidative destruction of heme in the absence of the contribution of antiperoxide enzymes (catalase, glutathione peroxidase, peroxiredoxin-2) in Н2О2 utilization. This raises the question of the independent AO significance of selenium in hemoglobin, in its protection from peroxide effects without the GPX mechanism of Н2О2 utilization.

Keywords

пероксид водорода селен селенит натрия флуоресценция глутатионпероксидаза эритроциты

Date of publication

24.10.2025

Year of publication

2025

Number of purchasers

Views

References

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GOST	Huseynov T., Kozlova M. Selenium as a Protector Against Hydrogen Peroxide Oxidative Degradation of Heme of Hemoglobin without the Glutathione Peroxidase Mechanism // Biophysics. – 2025. – V. 70. – Issue number 1 C. 104-111 . URL: https://biophysras.ru/s0006302925010124-1/?version_id=106193. DOI: 10.31857/S0006302925010124
MLA	Huseynov, T. M, Kozlova, M. V "Selenium as a Protector Against Hydrogen Peroxide Oxidative Degradation of Heme of Hemoglobin without the Glutathione Peroxidase Mechanism." Biophysics. 70.1 (2025).:104-111. DOI: 10.31857/S0006302925010124
APA	Huseynov T., Kozlova M. (2025). Selenium as a Protector Against Hydrogen Peroxide Oxidative Degradation of Heme of Hemoglobin without the Glutathione Peroxidase Mechanism. Biophysics. vol. 70, no. 1, pp.104-111 DOI: 10.31857/S0006302925010124

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Selenium as a Protector Against Hydrogen Peroxide Oxidative Degradation of Heme of Hemoglobin without the Glutathione Peroxidase Mechanism

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Selenium as a Protector Against Hydrogen Peroxide Oxidative Degradation of Heme of Hemoglobin without the Glutathione Peroxidase Mechanism

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