- PII
- S0006302925010162-1
- DOI
- 10.31857/S0006302925010162
- Publication type
- Article
- Status
- Published
- Authors
- Volume/ Edition
- Volume 70 / Issue number 1
- Pages
- 137-143
- Abstract
- Temperature heterogeneity of the electrophoretic solution, dynamically changing during electrophoresis in the gel platform area, was demonstrated by us earlier. In order to understand the reasons for this effect, we used U-shaped inserts in the wells of the electrophoretic chamber, protruding above the surface of the electrophoretic solution, but not affecting the current flow. The conducted study showed that the migration of heated masses of solution is caused by different rates of hydrogen and oxygen production on platinum electrodes. Using technique of real-time thermal imaging, it was demonstrated that in the presence of U-shaped inserts, heating was uniform up to 7°С throughout the entire volume of the solution located above the gel platform. Heating of the liquid in the well area occurred with less dynamics and was not identical. The heating of the liquid in the cathode well occurs insignificantly (only 1.5°C), and at the anode the increase is 2.5°С. We believe that temperature homogeneity will be of significant importance for reducing the variability of the results obtained by the Comet assay (DNA comet) method on preparations subjected to alkaline electrophoresis.
- Keywords
- нуклеоиды ДНК-кометы щелочной электрофорез ИК-термограммы
- Date of publication
- 24.10.2025
- Year of publication
- 2025
- Number of purchasers
- 0
- Views
- 16
References
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