- PII
- S0006302925020079-1
- DOI
- 10.31857/S0006302925020079
- Publication type
- Article
- Status
- Published
- Authors
- Volume/ Edition
- Volume 70 / Issue number 2
- Pages
- 285-294
- Abstract
- A comparative molecular investigation of the action of earlier little-studied phosphatidic acid and well-studied cardiolipin in their interaction with cytochrome C was carried out. Using spectrofluorimetry, close conformational changes in the cytochrome C active center were shown to occur during its interaction not only with cardiolipin, but also with phosphatidic acid. By means of an EPR assay, a significant enhancement of cytochrome C peroxidase activity during its interaction with cardiolipin and phosphatidic acid was demonstrated by formation of etoposide phenoxyl radical and luminol-dependent chemiluminescence. The assay indicated about 30% more pronounced changes in cytochrome C peroxidase activity in the presence of phosphatidic acid as compared to a similar effect of cardiolipin. At the same time, a close to intensity overwhelming recovery of the action of cardiolipin or phosphatidic acid was shown spectrophotometrically by cytochrome C reduction, which decreases by approximately 25% as compared to control phosphatidylcholinecontaining samples. Thus, phosphatidic acid can effectively change the cytochrome C confirmation and peroxidase activity as well effectively, and in peroxidase activity PA even surpasses cardiolipin. The obtained results may signify a high involvement of both cardiolipin and phosphatidic acid in the development of cytochrome С-mediated proapoptotic processes in mitochondria which, in its turn, may lay become foundation for a new direction in regulatory lipidomics.
- Keywords
- цитохром с механизмы апоптоза фосфолипиды кардиолипин фосфатидная кислота
- Date of publication
- 24.10.2025
- Year of publication
- 2025
- Number of purchasers
- 0
- Views
- 18
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