RAS BiologyБиофизика Biophysics

  • ISSN (Print) 0006-3029
  • ISSN (Online) 3034-5278

Effects of Macromolecular Crowding in Erythrocyte Cytoplasm

PII
S0006302925020098-1
DOI
10.31857/S0006302925020098
Publication type
Article
Status
Published
Authors
Volume/ Edition
Volume 70 / Issue number 2
Pages
305-313
Abstract
Using Raman spectroscopy, fluorescence spectroscopy, and time-correlated single-photon counting, it was shown that increasing the incubation temperature of human erythrocytes enhances the homogeneity of hemoglobin distribution in the cytoplasm and reduces globin molecule packing density. This likely results from the cellular “macromolecular crowding” effect. Observed conformational changes are specific to intracellular Hb and independent of the plasma membrane surface potential. Multiple pools of hemoglobin with varying structure and dynamics are proposed, they form clusters, creating heterogeneous hemoglobin distribution. The role of these effects in oxygen transport and regulatory signaling is discussed.
Keywords
гемоглобин эритроцит комбинационное рассеяние краудинг
Date of publication
24.10.2025
Year of publication
2025
Number of purchasers
0
Views
18

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