- PII
- S30345278S0006302925040052-1
- DOI
- 10.7868/S3034527825040052
- Publication type
- Article
- Status
- Published
- Authors
- Volume/ Edition
- Volume 70 / Issue number 4
- Pages
- 670-676
- Abstract
- It has been shown by 31P NMR spectroscopy that reducing the number of washes of rat erythrocytes significantly increases the stability of 2,3-diphosphoglycerate in these samples. The level of 2,3-diphosphoglycerate remains unchanged for 3.5 hours with a single wash and for 4 hours without washing. At the same time, with standard double washing, the content of 2,3-diphosphoglycerate decreases already after 2.5 hours. Analysis of its distribution between red blood cells and removed plasma, as well as analysis of the serum of one animal, showed that all 2,3-diphosphoglycerate remains in the suspension of red blood cells and is absent in plasma and serum. The results obtained should be taken into account when working with intact erythrocytes.
- Keywords
- 31P-ЯМР-спектроскопия 2,3-дифосфоглицерат эритроциты
- Date of publication
- 13.12.2025
- Year of publication
- 2025
- Number of purchasers
- 0
- Views
- 38
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