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RAS BiologyБиофизика Biophysics

  • ISSN (Print) 0006-3029
  • ISSN (Online) 3034-5278

Effect of Ethanol on Proliferation, Contraction and Migration of Vascular Smooth Muscle Cells from Rat Aorta

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PII
S30345278S0006302925040103-1
DOI
10.7868/S3034527825040103
Publication type
Article
Status
Published
Authors
V.K. Zhalimov
  • Affiliation: Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences
E.I. Meshcheryakova
  • Affiliation: Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences
L.G. Bobyleva
  • Affiliation: Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences
I.M. Vikhlyantsev
  • Affiliation: Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences
Yu.V. Gritsyna
  • Affiliation: Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences
Volume/ Edition
Volume 70 / Issue number 4
Pages
715-723
Abstract
The aim of the work is to study the effect of 24-hour effect of ethanol (50 and 100 mmol/L) on proliferation, contraction and migration of vascular smooth muscle cells (VSMCs) from rat thoracic aorta. The following age groups of rats were studied: early neonatal period of development (1-week-old rats), puberty age (5-month-old rats) and the initial period of aging (1-year-old rats). The following methods were used in the work: real-time PCR to assess the proliferation of VSMCs by comparing the relative amount of genomic DNA between the control and the experiment; wound healing assay to determine the migration of VSMCs; the collagen gel contraction assay to assess the contraction of VSMCs. Ethanol 50 mmol/L did not have effect on the studied parameters of VSMCs. Ethanol 100 mmol/L resulted in: increase of proliferation ~38% (р ≤0.01) and decrease the contraction ~12% (р ≤ 0.01) of VSMCs of rats of the early neonatal period of development; decrease the contraction ~7% (р ≤ 0.01) and proliferation ~21% (р ≤ 0.01) of VSMCs of rats of puberty age and animals of the initial period of aging, respectively. Thus, ethanol 100 mmol/L had different effects on VSMCs of rats depending on age group of animals. It is worth noting that VSMCs of rats of the early neonatal period of development are more sensitive to effects of ethanol.
Keywords
этанол пролиферативная миграционная сократительная активности гладкомышечные клетки аорты сократительный и пролиферативный фенотипы
Date of publication
13.12.2025
Year of publication
2025
Number of purchasers
0
Views
37

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