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RAS BiologyБиофизика Biophysics

  • ISSN (Print) 0006-3029
  • ISSN (Online) 3034-5278

Production of Polyclonal Antibodies Specific to Obscurin of the Long-Tailed Ground Squirrel : Problems and Prospects

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PII
S30345278S0006302925060033-1
DOI
10.7868/S3034527825060033
Publication type
Article
Status
Published
Authors
T.A. Uryupina
  • Affiliation: Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences
S.S. Popova
  • Affiliation: Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences
G.Z. Mikhailova
  • Affiliation: Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences
L.G. Bobyleva
  • Affiliation: Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences
A.D. Ulanova
  • Affiliation: Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences
A.G. Bobylev
  • Affiliation:
    Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences
    Scientific and Technological University "Sirius"
S.N. Udaltsov
  • Affiliation: Institute of Physicochemical and Biological Problems of Soil Science – separate subdivision of the Federal Research Center "Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences"
N.M. Zakharova
  • Affiliation: Institute of Cell Biophysics of the Russian Academy of Sciences – separate subdivision of the Federal Research Center "Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences"
I.M. Vikhlyantsev
  • Affiliation: Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences
Volume/ Edition
Volume 70 / Issue number 6
Pages
1068-1074
Abstract
The obtained polyclonal antibodies specific to the N-sequence (76–289 aa) of obscurin of the long-tailed ground squirrel () were used to study the isoform composition of this protein in two striated muscles: the myocardium of the left ventricle and M. gastrocnemius medialis. One low molecular weight isoform of obscurin with a molecular weight of 115 kDa has been identified. No other isoforms of obscurin, including giant A- and B-isoforms, have been detected. Seasonal changes in the levels of the low molecular weight obscurin isoform visualized in the cardiac and skeletal muscles of long-tailed ground squirrels were not observed. Further research could focus on sequencing the obscu gene of long-tailed ground squirrels and analyzing obscurin transcripts to obtain antibodies specific to the giant isoforms of this protein.
Keywords
гигантские белки саркомерного цитоскелета обскурин obscn длиннохвостый суслик гибернация
Date of publication
15.10.2025
Year of publication
2025
Number of purchasers
0
Views
31

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