- PII
- S30345278S0006302925060196-1
- DOI
- 10.7868/S3034527825060196
- Publication type
- Article
- Status
- Published
- Authors
- Volume/ Edition
- Volume 70 / Issue number 6
- Pages
- 1221-1229
- Abstract
- One of the key challenges in transplantology is the limited period of safe, hypothermic storage for a donor heart. A promising solution to extend this time is gas hypothermic preservation using mixtures of oxygen and carbon monoxide, or other gas agents. However, standardizing this approach requires selecting the conditions for cardiac arrest and blood removal before gas exposure. The goal of this study was to investigate the feasibility of using hypobaric gas-hypothermic preservation for rat hearts, in combination with the cardioplegic solution Normacor mixed with the plasma substitute Gelofusin. An experimental study was conducted on isolated rat hearts ( = 30). Functional (contractility, heart rate) and morphological parameters were evaluated after 12 and 24 hours of storage at 4°C and 6.5 atm pressure of a CO : O (1 : 1) gas mixture. Three preparation protocols were compared: perfusion with "Normacor + Gelofusin" solution at 37°C, perfusion with Custodiol solution at 4°C, and storage without perfusion. It was shown that the "Normacor + Gelofusin" combination ensures stable recovery of cardiac contractile function (~50% of native control) and morphological integrity after 12 and 24 hours. After 12 hours, the Normacor group demonstrated a statistically significant improvement in functional recovery compared to the Custodiol group. Histological analysis revealed moderate, potentially reversible ischemia-reperfusion injuries in all groups after 24 hours of exposure to a gaseous medium. The combination of Normacor and Gelofusin solutions, both allowed for clinical use in Russia, was found to be an effective means for pre-conservative preparation of organs for subsequent gas-hypothermic preservation. This finding suggests that this approach could be introduced into clinical practice to extend the preservation time of donor hearts.
- Keywords
- нормакор кустодиол консервирующий раствор монооксид углерода хранение сердце трансплантология холодовая консервация
- Date of publication
- 15.10.2025
- Year of publication
- 2025
- Number of purchasers
- 0
- Views
- 39
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